Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources

  • Vesna Hadži-Tašković Šukalović Institute for Multidisciplinary Research, Kneza Višeslava 1, 11030 Belgrade
  • Mirjana Vuletić Maize Research Institute “Zemun Polje”, Slobodana Bajića 1, 11085 Belgrade
  • Ksenija Marković Maize Research Institute “Zemun Polje”, Slobodana Bajića 1, 11085 Belgrade
  • Željko Vučinić Institute for Multidisciplinary Research, Kneza Višeslava 1, 11030 Belgrade
  • Natalija Kravić Maize Research Institute “Zemun Polje”, Slobodana Bajića 1, 11085 Belgrade
Keywords: nitrate, ammonia, antioxidant enzymes, phenolic compounds


Antioxidant systems of maize root cell walls grown on different nitrogen sources were evaluated. Plants were grown on a medium containing only NO3- or the mixture of NO3-+NH4+, in a 2:1 ratio. Eleven-day old plants, two days after the initiation of lateral roots, were used for the experiments. Cell walls were isolated from lateral roots and primary root segments, 2-7 cm from tip to base, representing zones of intense or decreased growth rates, respectively. Protein content and the activity of enzymes peroxidase, malate dehydrogenase and ascorbate oxidase ionically or covalently bound to the walls, as well as cell wall phenolic content and antioxidant capacity, were determined. Cell walls of plants grown on mixed N possess more developed enzymatic antioxidant systems and lower non-enzymatic antioxidant defenses than cell walls grown on NO3-. Irrespective of N treatment, the activities of all studied enzymes and protein content were higher in cell walls of lateral compared to primary roots. Phenolic content of cell walls isolated from lateral roots was higher in NO3--grown than in mixed N grown plants. No significant differences could be observed in the isozyme patterns of cell wall peroxidases isolated from plants grown on different nutrient solution. Our results indicate that different N treatments modify the antioxidant systems of root cell walls. Treatment with NO3- resulted in an increase of constitutive phenolic content, while the combination of NO3-+NH4+ elevated the redox enzyme activities in root cell walls.


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How to Cite
Hadži-Tašković ŠukalovićV., VuletićM., MarkovićK., Vučinić Željko, & KravićN. (2017). Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources. Spanish Journal of Agricultural Research, 14(4), e0808.
Plant physiology