Inhibitory effects of salicylic acid on Meloidogyne javanica reproduction in tomato plants

  • Fatemeh Moslemi University of Shahid Beheshti, Faculty of Biological Sciences. Tehran
  • Seddigheh Fatemy Iranian Research Institute of Plant Protection, Nematology Dept. P.O. Box 1454-19395, Tehran
  • Françoise Bernard University of Shahid Beheshti, Faculty of Biological Sciences. Tehran
Keywords: induced resistance, seed priming, soil drench, root-knot nematode


Root-knot nematodes (Meloidogyne spp.), play a major role in loss of agricultural production. Natural substances, such as salicylic acid (SA) could possibly be involved in inducing host plant resistance against nematodes. The present study is concerned with exploring the effects of varying concentrations of SA as seed priming and soil drench on tomato growth parameters and the reproduction of the root-knot nematode Meloidogyne javanica. SA at 50 μM concentration caused only 2% of juvenile mortality under in vitro conditions. SA applied as 50 μM seed treatment caused 95% and, as a soil drench, 78% reduction in the number of egg masses that formed on tomato plants. The numbers of galls were reduced to a lesser extent. Final nematode density per gram of soil was reduced to less than 1 by the 50 μM SA seed treatment, and in other treatments decreased by between 70 and 88% compared with control plants. Our results indicate SA has potential to lower root knot nematode reproduction in tomato, and seed priming is a fairly easy method to work with.


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Adrian M, Trouvelot S, Gamm M, Poinssot B, Héloir M, Daire X, 2012. Mechanism and signal transduction: Theoretical and applied approaches. In: Plant defense: biological control; Merillon JM, Ramawat KG (eds). pp: 313-331. Springer, Dordrecht.

Akhyani A, Mojtahedi H, Naderi A, 1984. Species and physiological races of root-knot nematodes in Iran. Iran J Plant Pathol 20: 57-71.

Bednarek P, Osbourn A, 2009. Plant-microbe interactions: chemical diversity in plant defense. Science 324: 746-748.

Benhamou N, Nicole M, 1999. Cell biology of plant immunization against microbial infection: the potential of induced resistance in controlling plant diseases. Plant Physiol Biochem 37: 703-710.

Branch C, Hwang, CF, Navarre DA, Williamson VM, 2004. Salicylic acid is part of the Mi-1-mediated defense responses to root-knot nematode in tomato. Mol Plant-Microbe Interact 17: 351-356.

Dempsey MA, Klessig DF, 2012. SOS - too many signals for systemic acquired resistance. Trends Plant Sci 17 (9): 538-545.

Ding F, Dokholyan NV, Buldyrev SV, Stanley HE, Shakhnovich EI, 2002. Direct molecular dynamics observation of protein folding transition state ensemble. Biophys J 83: 3525-3532.

Durrant WE, Dong X, 2004. Systemic acquired resistance. Annu Rev Phytopathol 42: 185-209.

Fraissinet-Tachet L, Baltz R, Chong J, Kauffmann S, Fritig B, Saindrenan P, 1998. Two tobacco genes induced by infection, elicitor and salicylic acid encode glucosyl transferases acting on phenylpropanoids and benzoic acid derivatives, including salicylic acid. Fed Eur Biochem Soc Lett 437: 319-323.

Fu ZQ, Dong X. 2013. Systemic acquired resistance: turning local infection into global defense. Annu Rev Plant Biol 64: 839-863.

Fu ZQ, Yan S, Saleh A, Wang W, Ruble J, Oka N, Mohan R, Spoel SH, Tada Y, Zheng N, Dong X, 2012. NPR3 and NPR4 are receptors for the immune signal salicylic acid in plants. Nature 486: 228-233.

Hammerschmidt R, Kuc J, 1995. Induced resistance in plants. Kluwer Academic, Dordrecht. 182 pp.

Hartman KM, Sasser JN, 1985. Identification of Meloidogyne species on the basis of differential host test and perineal pattern morphology. In: An advanced treatise on Meloidogyne, Vol. II; Barker KR, Carter, CC, Sasser JN (eds). pp: 69-77. Graphics, North Carolina State University, USA.

Jones JD, Dangl JL, 2006. The plant immune system. Nature 444: 323-329.

Kempster V, Davies KA, Scott E, 2001. Chemical and biological induction of resistance to the clover cyst nematode (Heterodera trifolii) in white clover (Trifolium repens). Nematology 3(1): 35-43.

Kessler A, Baldwin IT, 2002. Plant responses to insect herbivory: the emerging molecular analysis. Annu Rev Plant Biol 53: 299-328.

Lin J, Mazarei M, Zhao N, Zhu NJ, Zhuang X, Liu W, Pantalone VR, Arelli PR, Stewart Jr CN, 2013. Overexpression of a soybean salicylic acid methyltransferase gene confers resistance to soybean cyst nematode. Plant Biotech J 11 (9): 1135-1145.

Mahdikhani A, Kheiri A, Mohammadi M, Eshtiagh H, Okhovat M, 2003. The introduction of three new species of the genus, Meloidogyne, for Iran. J Plant Dis Prot 39: 189-211.

Mende A, Mosch J, ZellerW, 1993. Untersuchungen zur Resistenzinduktion durch ausgewählte Pflanzenextrakte gegen den Feuerbrand (Erwinia amylovora (Burrill) Winslow et al.). Z Pflanzenkrankh Pflanzenschutz 101: 141-147.

Molinari S, 2008. Salicylic acid as an elicitor of resistance to root-knot nematodes in tomato. Acta Hortic 789: 119-126.

Mostafanezhad H, Sahebani N, NourinejhadZarghani S, 2014. Control of root-knot nematode (Meloidogyne javanica) with combination of Arthrobotrys oligospora and salicylic acid and study of some plant defense responses. Biocontrol Sci Tech 24 (2): 203-215.

Mukherjee A, Babu SS, Mandal F, 2012. Potential of salicylic acid activity derived from stress-induced (water) tomato against Meloidogyne incognita. Arch Phytopathol Plant Prot 45: 1909-1916.

Nandi B, Sukul NC, Banerjee N, Sengupta S, Das P, SinhaBabu SP, 2002. Salicylic acid enhances resistance in cowpea against Meloidogyne incognita. Phytopathol Mediterr 41: 39-44.

Nandi B, Kundu K, Banerjee N, Babu SPS, 2003. Salicylic acid-induced suppression of Meloidogyne incognita infestation of okra and cowpea. Nematology 5: 747-752.

Nishimura MT, Dangl JL, 2010. Arabidopsis and the plant immune system. Plant J 61: 1053-1066.

Ohashi Y, Ohshima M, 1992. Stress-induced expression of genes for pathogenesis-related proteins in plants. Plant Cell Physiol 33: 819-826.

Oka Y, Cohen Y, 2001. Induced resistance to cyst and root-knot nematodes in cereals by DL-ß-amino butyric acid. Eur J Plant Pathol 107: 219-227.

Oka Y, Cohen Y, Spiegel Y, 1999. Local and systemic induced resistance to the root-knot nematode in tomato by DL-ß-amino butyric acid. Phytopathology 89: 1138-1143.

Pankaj NA, Shakil HK, Chawla S, Gaur HS, 2005. Effect of phenolic acids on root-knot nematode, Meloidogyne incognita infesting cowpea. Int J Nematol 15: 238-242.

Ryals J,Uknes S, Ward, E, 1994. Systemic acquired resistance. Plant Physiol 104: 1109-1112.

Shah J, 2009. Plants under attack: systemic signals in defence. Curr Opin Plant Biol 12: 459-464.

Sikora RA, Fernandez E, 2005. Nematode parasites of vegetables. In: Plant parasitic nematodes in subtropical and tropical agriculture; Luc M, Sikora RA, Bridge J (eds), 2nd edition. pp: 319-392. CABI Publishing.

Sticher L, Mauch-Mani B, Metraux JP, 1997. Systemic acquired resistance. Annu Rev Phytopath 35: 235-270.

Thulke O, Conrath U, 1998. Salicylic acid has a dual role in the activation of defense-related genes in parsley. The Plant Journal 14: 35-42.

Van Loon LC, Rep M, Pieterse CMJ, 2006. Significance of inducible defence-related proteins in infected plants. Annu Rev Phytopath 44: 135-162.

Vlot AC, Dempsey DA, Klessig DF, 2009. Salicylic acid, a multifaceted hormone to combat disease. Annu Rev Phytopathol 47: 177-206.

Whitehead AG, Hemming JR, 1965. A comparision of some quantitative methods of extracting small vermiform nematodes from soil. Ann Appl Biol 55: 25-38.

Zeller W, 2006. Status on induced resistance against plant bacteria diseases. Fitosanidad 10 (2): 99-103.

How to Cite
MoslemiF., FatemyS., & BernardF. (2016). Inhibitory effects of salicylic acid on Meloidogyne javanica reproduction in tomato plants. Spanish Journal of Agricultural Research, 14(1), e1001.
Plant protection